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. 2019 Dec 24;9:19767. doi: 10.1038/s41598-019-56365-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Decreased T_FR cells in autoantibody-positive AIHA mice. (A) The expression of CD25 and Foxp3 in CXCR5⁺CD4⁺T cells from mice in the responder, non-responder, and control groups. (B) The percentages of CD25⁺Foxp3⁺ cells in CXCR5⁺CD4⁺T cells in these three groups. (C) The ratio of T_FH:T_FR in these three groups. (D) Relationship of T_FH:T_FR and the fluorescent units of IgG-specific autoantibodies in responder the group. (E) The number of CD4⁺CXCR5⁺CD25⁺FoxP3⁺T_FR cells in spleen in these three groups. Experiments were repeated three times with 3–4 mice for each group. Data shown were the mean ± SEM. *p < 0.05; ns, no significance.