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. 2019 Dec 24;9:19767. doi: 10.1038/s41598-019-56365-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The equal functionality of T_FH cells between the responder and non-responder. CXCR5⁺CD4⁺CD25⁻T_FH cells sorted from the responder group and non-responder group in week 12 was cultured with CD19⁺ B cells from the responder group along with anti-CD3 and anti-IgM for six days. B cells were intracellularly stained for IgG1 and surface stained for GL7. The percentage of IgG1⁺(A) andGL7⁺ (B) cells was calculated in CD19⁺ B cells. Data were from three independent experiments and the mean ± SEM were shown. ns, no significance