Table 3.
Purification of two molecular forms of AChE from the brain of fish T. mossambica.
| Stage | Volume (mls) | Total AChE (µmol/min) | Total protein (mgs) | Sp. Activity Units/mg | Fold Purification | Yield % |
|---|---|---|---|---|---|---|
| Homogenate | 71.30 | 147.05 | 429.50 | 0.3424 | 1.00 | 100.00 |
| Triton-solubilzed fraction | 70.00 | 97.65 | 268.90 | 0.3631 | 1.06 | 66.41 |
| 50% satd. (NH4)2SO4 fraction | 64.00 | 69.88 | 166.54 | 0.4196 | 1.22 | 47.52 |
| Con A Sepharose eluate | 5.20 | 10.60 | 2.14 | 4.9890 | 14.57 | 7.21 |
| Preparative PAGE: | ||||||
| Band I | 44.00 | 0.55 | 0.61 | 0.9006 | 2.63 | 0.37 |
| Band II | 92.00 | 3.84 | 0.61 | 6.3100 | 18.43 | 2.61 |
| Total Yield: | 2.98 | |||||