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. 2019 Dec 24;9:19762. doi: 10.1038/s41598-019-56066-x

Table 4.

Km (mM ATChI) and Vmax (µmol/min/g protein) of Bands I and II AChE at different assay temperatures.

Assay Band I Band II
temp Km Vmax Km Vmax
10 °C 0.4 ± 0.014 0.0073 ± 0.000 0.21 ± 0.007 0.0323 ± 0.001
15 °C 2.0 ± 0.070 0.0255 ± 0.001 0.14 ± 0.005 0.042 ± 0.001
20 °C 2.4 ± 0.084 0.0508 ± 0.002 0.21 ± 0.007 0.0584 ± 0.002
25 °C 5.38 ± 0.188 0.1691 ± 0.006 0.17 ± 0.006 0.0721 ± 0.003
30 °C 2.5 ± 0.088 0.1568 ± 0.005 0.27 ± 0.009 0.1097 ± 0.004
35 °C 8.42 ± 0.295 0.6154 ± 0.022 0.36 ± 0.013 0.1466 ± 0.005
40 °C 4.54 ± 0.159 0.5752 ± 0.020 0.52 ± 0.018 0.208 ± 0.007