Table 4.
Km (mM ATChI) and Vmax (µmol/min/g protein) of Bands I and II AChE at different assay temperatures.
| Assay | Band I | Band II | ||
|---|---|---|---|---|
| temp | Km | Vmax | Km | Vmax |
| 10 °C | 0.4 ± 0.014 | 0.0073 ± 0.000 | 0.21 ± 0.007 | 0.0323 ± 0.001 |
| 15 °C | 2.0 ± 0.070 | 0.0255 ± 0.001 | 0.14 ± 0.005 | 0.042 ± 0.001 |
| 20 °C | 2.4 ± 0.084 | 0.0508 ± 0.002 | 0.21 ± 0.007 | 0.0584 ± 0.002 |
| 25 °C | 5.38 ± 0.188 | 0.1691 ± 0.006 | 0.17 ± 0.006 | 0.0721 ± 0.003 |
| 30 °C | 2.5 ± 0.088 | 0.1568 ± 0.005 | 0.27 ± 0.009 | 0.1097 ± 0.004 |
| 35 °C | 8.42 ± 0.295 | 0.6154 ± 0.022 | 0.36 ± 0.013 | 0.1466 ± 0.005 |
| 40 °C | 4.54 ± 0.159 | 0.5752 ± 0.020 | 0.52 ± 0.018 | 0.208 ± 0.007 |