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. 2019 Dec 24;9:19812. doi: 10.1038/s41598-019-56231-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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MitosRNA-tRNA-cys sequences are present and induced in response to anoxia in the A. limnaeus WS40NE cell line. (a) Heat map of mitosRNAs differentially expressed in response to anoxia in WS40NE cells. Log₂ fold change is calculated relative to normoxic control with fetal bovine serum (N + FBS). For heat map details see Fig. 1 caption. (b) MitosRNAs-tRNA-cys 3′ variants northern blot of normoxic and 24 h anoxic WS40NE cells shows increased expression of a variety of mitosRNAs derived from tRNA-cys during anoxia. 20 µg RNA was loaded for normoxic and anoxic cells and the blot was hybridized with 2 nM of mitosRNA-tRNA-cys probe. Note: smear of RNA at top of blot is due to need to load high amount of RNA in order to get a signal for the mitosRNAs. This is likely due to relatively low expression of mitosRNAs in the cells. (c) Line graphs of the 6 most highly differentially expressed mitosRNAs in response to anoxia. Note appearance of 3 variants of mitosRNAs annotating to mitochondrial tRNA-cys.