Table 3.
Sequences of the primers used in real-time PCR analysis.
| Gene name (Symbol) | Gene bank accession no. | Primer | Sequence (5′-3′) | Tm, °C | Length (bp) |
|---|---|---|---|---|---|
| β-actin (ACTB)* | NM_173979.3 |
Forward Reverse |
AGCAAGCAGGAGTACGATGAGT ATCCAACCGACTGCTGTCA |
60.5 58.0 |
120 |
| Lysyl oxidase (LOX) | NM_173932.4 |
Forward Reverse |
AACAATGTCGTCCGCTGTGA CCTTTGGGAGTTTTGGCTTGC |
60.2 60.5 |
102 |
| Peroxisome proliferator activated receptor gamma (PPARG) | NM_181024.2 |
Forward Reverse |
AATCCCTGTTCCGTGCTGTG AAAGTTGGTGGGCCAAAACG |
59.6 58.9 |
149 |
| Adiponectin | NM_174742.2 |
Forward Reverse |
CCCTGACTGAAGTCTGTGGC TCTTCCATGTTGTCCTCGCC |
60.3 60.0 |
115 |
| Acetyl-CoA carboxylase alpha (ACC) | NM_174224.2 |
Forward Reverse |
AGACGTTGGAAGCAGAGAGG TTCAGCTCCAGAGGTTTGGC |
59.4 55.0 |
142 |
| Fatty acid binding protein 4 (FABP4) | NM_174314.2 |
Forward Reverse |
GCTGCACTTCTTTCTCACCT TTCCTGGTAGCAAAGCCCAC |
58.1 60.3 |
140 |
| Troponin C1, slow skeletal and cardiac type (TNNC1) | NM_001034351.2 |
Forward Reverse |
GTGAGCCGCCAGTATGGATG CACGAAGATGTCAAAGGCCG |
60.9 59.6 |
97 |
| Troponin T1, slow skeletal type (TNNT1) | XM_010815469.3 |
Forward Reverse |
GCCTCTGAACATCGACCACA AGCTTCGCCATCAGGTCAAA |
55.0 60.0 |
111 |
| Myoglobin (MB) | NM_173881.2 |
Forward Reverse |
GAGTCACATGCCAACAAGCAC GAAGTCTGAAGGATGCTTGGC |
60.3 59.2 |
99 |
| Myosin heavy chain 2 (MYH2) | NM_001166227.1 |
Forward Reverse |
AAGAGCCCTTGGAATGAGGC ATGGCCATTTCCTGGTCCG |
60.0 60.1 |
138 |
| Myosin, heavy chain 4, skeletal muscle (MYH4) | NM_174224.2 |
Forward Reverse |
GGTCCAAGTGCTGAAGAGGG GATGCAGCGTACAAAGTGGG |
60.3 59.6 |
150 |
| Citrate synthase (CS) | NM_001044721.1 |
Forward Reverse |
CCATGGCTTTACTCACTGCG TTCGTGGAAGAAGCACTGGC |
59.3 60.9 |
97 |
| Isocitrate dehydrogenase (NADP(+)) 1 (IDH1) | NM_181012.3 |
Forward Reverse |
TCCGAAATATCCTGGGTGGC CCCTGGCACAACAAAATCGG |
59.5 60.0 |
149 |
| Hexokinase 2 (HK2) | XM_015473383.2 |
Forward Reverse |
TGCTCGCCTACTTCTTTACGG CCATCTCCTTGCGAAAACGC |
60.1 60.2 |
126 |
| Phosphofructokinase, muscle (PFKM) | NM_001075268.1 |
Forward Reverse |
GGACAATCTGCAAAGAAGCCC CCACCAGAGGTTAACACGGC |
52.4 60.0 |
126 |
| Phosphoglycerate kinase 1 (PGK1) | NM_001034299.1 |
Forward Reverse |
GTGGAGGAAGAAGGGAAGGG GAAAGTGAAGCTCGGAAGGC |
59.4 59.2 |
89 |
| Lactate dehydrogenase A (LDHA) | NM_174099.2 |
Forward Reverse |
GCTATTAATCGGTGCCCCAGG TTGCCATCTTGGACTTAGACCC |
60.9 60.0 |
110 |
*ACTB = Internal control gene.