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. 2019 Dec 24;9:19746. doi: 10.1038/s41598-019-56327-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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MicroRNA-548 cluster can regulate expression of HMBG1 in human amniotic epithelial cells (hAECs). (A) Levels of miRNA-548aa, miRNA-548ai, miRNA-548a-3p, and miRNA-548x-5p in hAECs transfected with miRNA-548aa inhibitor, miRNA-548ai inhibitor, miRNA-548a-3p inhibitor, miRNA-548x-5p inhibitor, and control inhibitor. (B) HMGB1 mRNA and protein expression in hAECs transfected with miRNA-548 inhibitor and with control inhibitor. (C) HMGB1 levels in culture medium of hAECs transfected with miRNA-548 inhibitor and with control inhibitor. **Significant difference (P < 0.01) compared with control group. hAECs, human amniotic epithelial cells.