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. 2019 Dec 24;9:19748. doi: 10.1038/s41598-019-56242-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Glycated collagen 1 regulates adipocyte insulin signaling and Rho and AGE scavenger receptor expression. Preadipocytes from human VAT were differentiated into mature adipocytes in 2D culture +/− unglycated or glycated recombinant human collagen 1 (UC1, GC1), then studied with glucose uptake assay or RT-qPCR. (a) Glucose uptake in non-stimulated (basal) or insulin-stimulated conditions. Ordinate: mean glucose uptake measured by ³H-2D-glucose in cell lysates (cpm) normalized to cell lysate protein concentration (mg/ml); bars with different letters indicate P < 0.050; n = 15 NDM, 15 DM subjects. (b) Gene expression studied with RT-qPCR. Ordinate: mean fold difference in transcript level in GC1 arm relative to UC1 arm referent = 1; *P < 0.050, comparing transcript levels in GC1 arm vs. UC1 arm; n = 12 NDM, 12 DM subjects.