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. 2019 Dec 24;9:19797. doi: 10.1038/s41598-019-56442-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fecal commensal microbiota composition following fecal microbiota transplantation in C. jejuni infected secondary abiotic mice. Secondary abiotic mice were infected with C. jejuni on days 0 and 1 by gavage. Starting a week later, infected mice were subjected to peroral fecal microbiota transplantation (FMT) from murine donors (open circles; n = 16) on three consecutive days or received vehicle (mock; closed circles; n = 13). On day 21 post-infection (i.e., 14 days post FMT), the fecal commensal microbiota composition was surveyed by culture-independent 16S rRNA methods quantitating the main bacterial groups and species including enterobacteria (EB), enterococci (EC), lactobacilli (LB), bifidobacteria (BB), Bacteroides/Prevotella (BP) species, Clostridium coccoides group (CC), Clostridium leptum group (CL) and Mouse Intestinal Bacteroides (MIB) and expressed as gene numbers per ng DNA. Medians and significance levels (p-values; **p < 0.01; ***p < 0.001) determined by the Mann-Whitney U test are given. Data were pooled from three independent experiments.