Figure 5.

Colonic immune cells responses upon fecal microbiota transplantation of C. jejuni infected secondary abiotic mice. Secondary abiotic mice were infected with C. jejuni on days 0 and 1 by gavage. Starting a week later, infected mice were subjected to peroral fecal microbiota transplantation (FMT) from murine donors (open circles) on three consecutive days or received vehicle (mock; closed circles). On day 21 post-infection (i.e., 14 days post FMT), the average numbers of (A) macrophages and monocytes (F4/80+), (B) T lymphocytes (CD3+), (C) regulatory T cells (FOXP3+), and (D) B lymphocytes (B220+) in the large intestinal mucosa and lamina propria were determined from six high power fields (HPF, 400 x magnification) per mouse in immunohistochemically stained large intestinal paraffin sections. Naive secondary abiotic mice served as uninfected and untreated controls (open diamonds). Medians, numbers of analyzed animals (in parentheses) and significance levels (p-values) determined by the Mann-Whitney U test are given. Data were pooled from three independent experiments.