Extended Data Fig. 9. Phenotypes of overexpression of RITF1 in plt2.
(a) NBT stained roots with or without XVE-RITF1 in Col and plt2 24 h after treatment with mock or 10 μM Estradiol. (b) Quantification of NBT staining intensity in the differentiation zone with or without XVE-RITF1 in Col and plt2. (n = 8 independent roots, *p < 5.5E-06). (c) Confocal images of roots with or without XVE-RITF1 in Col and plt2 24 h after mock or 10 μM estradiol treatment stained with PI. (d) The number of cells in the meristematic zone with or without XVE-RITF1 in Col and plt2 24 h after mock or 10 μM estradiol treatment. (n = 7 independent roots, *p < 4.5E-05). All scale bars = 50 μm. White and blue arrowheads indicate junction between the meristematic and elongation zones. Bar graphs represent mean. Error bars are ± SD. Dots indicate each data point. P values calculated by two-sided Student’s t-test.
