Figure 2. Expression of RITF1 and phenotype of RITF1 overexpression line.

(a) Expression of RITF1 in meristematic zone 1 h after 100 nM RGF1 treatment measured by RNA-seq (CPM, counts per million mapped reads) (n = 3 independent experiments, p < 0.01) (b) Expression of RITF1 in developmental zones measured by RNA-seq (FPKM, Fragments Per Kilobase of transcript per Million mapped reads). (c) Expression of RITF1 in meristematic zone of wild type and rgfr1/2/3 upon RGF1 treatment measured by qRT-PCR. (n = 3 independent experiments, *p<0.001, **p<0.002, and ***p<0.02). (d) Confocal images of pRITF1-GFP and PI stained root from wild type and the rgfr1/2/3 after RGF1 treatment. (e) Total intensity (arbitrary unit A.U.) of pRITF1-GFP expression in wild type and rgfr1/2/3 24 h after RGF1 treatment (n = 5 independent roots, *p < 0.001). Confocal images of roots stained with PI (f) and H₂O₂-BES-Ac (g) in Col-0 and XVE-RITF1 in Col-0 after mock or Estradiol treatment. (h) Light microscope images of NBT stained roots after mock or Estradiol treatment. (i) Number of cells in the meristematic zone in Col-0 and XVE-RITF1 after mock or Estradiol treatment. (n = 6 independent roots *p < 0.001). (j) Average intensity of BES-H₂O₂-Ac in differentiation zone after mock or Estradiol treatment (n = 6 independent roots, *p < 0.001). (k) Average intensity of NBT staining in differentiation zone after mock or Estradiol treatments (n = 7 independent roots, *p < 0.001). Scale bar = 50 μm. White and blue arrowheads indicate junction between meristematic and elongation zones and between elongation and differentiation zones. Bar graphs represent mean. Error bars are ± SD. Dots indicate each data point. P values calculated by two-sided Student’s t-test.