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. Author manuscript; available in PMC: 2021 Feb 1.
Published in final edited form as: Biomaterials. 2019 Nov 18;230:119634. doi: 10.1016/j.biomaterials.2019.119634

Figure 3: Cell contractility partly contributes to construct stiffening.

Figure 3:

Hydrogel constructs with either 27 or 32 mg/mL PEGVS were cultured for 6 d and then were incubated with 30 μM blebbistatin or vehicle during the final 24 h of culture. The shear storage modulus (G’) at 7 d was measured in each condition (A). Average stiffness at 1 d is shown in red on each graph for reference. 24 h treatment with blebbistatin did not affect vessel density (B), the cell density (C), or cell area covered (D). Blebbistatin induced a slight increase in cell circularity in 27 mg/mL PEGVS constructs (E). Blebbistatin did not alter the density of microbeads (F). *: p < 0.05, relative to vehicle control, N=3.