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. Author manuscript; available in PMC: 2021 Feb 1.
Published in final edited form as: Biomaterials. 2019 Nov 18;230:119634. doi: 10.1016/j.biomaterials.2019.119634

Figure 5: Collagen I alpha 1 chain expression is upregulated in soft hydrogels.

Figure 5:

RNA was collected from constructs after 1 d and 7 d, purified, and expression determined using real time RT-PCR. Expression of collagen I α1 chain (COL1A1, A), fibronectin (FN1, B), laminin β1 chain (LAMB1, C), and collagen IV α1 chain (COL4A1, D) was measured. Expression levels were normalized to glyceraldehyde 3-phosphate dehydrogenase (GAPDH) in each sample and then to expression in 27 mg/mL PEGVS at 1 d (ΔΔCt method). *: p < 0.05, relative to 40 mg/mL PEGVS same day, N=3.