Extended Data Figure 9. Increased MCT1 expression in melanomas is associated with significantly worse patient survival. Related to Figure 2.

a-d, Kaplan-Meier overall survival curves of melanoma patients stratified based on MCT1 (a), MCT2 (b), MCT4 (c), and CD147 (d) expression level within tumor specimens. Data were from the SKCM cohort in TCGA (https://portal.gdc.cancer.gov/projects/TCGA-SKCM). Each panel compares the top third of patients with the highest expression levels versus the bottom third of patients with the lowest expression levels. Ticks represent censored values. e-f, Flow cytometry plots showing the gating strategies used to identify human melanoma cells in subcutaneous tumors (e) or the blood (f) of xenografted mice. Cells were gated on forward versus side scatter (FSC-A vs. SSC-A) to exclude red blood cells and clumps of cells. Human melanoma cells were selected by including cells that stained positively for DsRed (stably expressed in all melanoma lines) and HLA and excluding cells that stained positively for the mouse hematopoietic and endothelial markers CD45, CD31, or Ter119. The statistical significance of the differences in overall survival (a-d) were assessed using the Mantel-Cox log-rank test.