Extended Data Figure 2. Efficient metastasizers express higher levels of MCT1 than inefficient metastasizers. Related to Figure 2.

a, Quantification of MCT1 relative to Actin bands from the western blot in Figure 2a comparing efficient versus inefficient metastasizers. b, Quantification of MCT4 relative to Actin bands from the western blot in Figure 2c comparing efficient versus inefficient metastasizers. c-d, Quantification of mean fluorescence intensities for MCT1 staining in the flow cytometry plots comparing efficient (Fig. 2e) and inefficient (Fig. 2d) metastasizers. HCC15 cells and MCT1-deficient HCC15 cells were positive and negative controls (c). e-f, Immunofluorescence staining for MCT1 (green) in sections from subcutaneous tumors from inefficiently (e, UM47) or efficiently (f, M405) metastasizing melanomas. An adjacent section was stained with an antibody against S100b (a melanoma marker, green). Images are representative of three independent experiments per melanoma. g,h Immunofluorescence staining for MCT1 (green) in sections from subcutaneous tumors from inefficient (g, M498, M610, and M597) and efficient (h, M481, UT10, and M405) metastasizers. In each case, an adjacent section was stained with an antibody against S100b (a melanoma marker, green). Images are representative of results from two independent experiments per melanoma. i-j, While efficient metastasizers often exhibited cell surface staining (j), inefficient metastasizers typically exhibited diffuse cytoplasmic staining (i). Images are representative of results from two independent experiments per melanoma. All data represent mean ± s.d.. Statistical significance was assessed using Student’s t-tests (a-b and d).