Figure 6.

In vitro splicing assay. Mini-gene constructs containing one or two human pendrin exons with flanking introns (150-bp) were transcribed in HEK293T cells. The transcripts were reverse transcribed and amplified by PCR. Omission (skipping) of an exon(s) results in 190-bp band as pET01vector control does. Successful retention of an exon(s) produces a larger band depending on the size of the exon(s). Pendrin variants that produced clearly detectable misspliced band(s) are enclosed by gray squares. The DNA sequences of the mini-gene constructs are provided in Supporting Information.