Figure 3.

Effect of oral DBP exposure on ovarian follicle p-H2AX. Adult cycling female CD-1 mice were treated daily with vehicle or DBP and their ovaries processed for immunofluorescence as described in Materials and Methods. Representative images (from n = 3 mice per treatment) show p-H2AX (green), DAPI (blue), or merged staining in ovarian follicles of mice treated with vehicle control (VEH), 10 μg/kg/day (DBP10), 100 μg/kg/day (DBP100), 1000 μg/kg/day (DBP1000), or a no primary antibody negative control (negative). Images were taken at 200× magnification. Scale bar represents 100 μm. Arrows show large antral follicles, and arrowheads point at small follicles.