Figure 6.

Effect of oral DBP exposure on Brca1 promoter methylation. Adult cycling female CD-1 mice were treated daily with vehicle or DBP and their ovaries processed for DNA methylation or qPCR analysis as described in Materials and Methods. Methylation data are presented as mean percent methylated ± SEM. Panel A shows methylation data for the cleavage sites CpG1 to 3 and CpG6 to 8, while panel B shows data for sites CpG14 to 18–20 and CpG22–23. Panel C shows mean relative expression for the DNA methyltransferase Dnmt1. Data were compared between treatments using Kruskal-Wallis nonparametric followed by Mann-Whitney post hoc test with significance set at p ≤ 0.05 (n = 5–7 mice per treatment).