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. 2020 Jan 1;16(1):12–26. doi: 10.7150/ijbs.37269

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The effects of Andr on Cardiac fibroblast in vitro. Cardiac fibroblasts were treatment with or without tri-gas incubator (Panasonic,Japan) and treated with different concentrations of Andr (0, 12.5, 25, or 50 µM). A and B. Immunofluorescence staining of α-SMA and quantitative analysis of fluorescence in the indicated groups. C-E. Representative blots of TGFβ, and phosphorylated (P-) and total (T-) smad3 in the cardiac fibroblasts (C) and quantitative analysis (D-E) in the indicated groups (n = 6). F-H. The mRNA levels of collagen I, Fibronectin and CTGF in cardiac fibroblasts in the indicated groups (n = 6). The results were presented as a fold change, and the data are given as the mean ± SEM.∗ P < 0.05 compared with the control group. # P < 0.05 vs. the hypoxia without Andr group.