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. 2020 Jan 1;16(1):12–26. doi: 10.7150/ijbs.37269

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Andr alleviated oxidative stress after myocardial infarction in vivo. A and B. Immunohistochemical staining of 4-hydroxynonenal in the indicated groups 3 weeks post-MI surgery or sham (A) and the relevant method for quantitative analysis (B) in mouse hearts. (n=6 per groups). C-E. Western blot analysis of SOD2, Gp91 among different groups (C) and quantitative analysis (D,E) in the hearts (n=6 per groups). F and G. DHE staining performed in the indicated groups and quantitative analysis of fluorescence by Image Pro Plus. H-M. Real-time PCR analysis of oxidative markers (Gp91, NADPH P67 phox and NOX4) and anti-oxidant makers (Gpx, SOD2 and NQO1). The data are given as the mean ± SEM. *P<0.05 vs vehicle-Sham; #P<0.05 vs vehicle-MI.