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. 2020 Jan 1;16(1):99–115. doi: 10.7150/ijbs.35643

Figure 4.

Figure 4

Wnt/β-catenin pathway mediates oncogenic activity of SRA in ECC-1 cells. (A) Expression levels of β-catenin, Gsk-3β, and h-cMyc mRNAs were measured in ECC-1 cells transfected with siSRA or siNC. (B) Expression levels of β-catenin, Gsk-3β, and h-cMyc mRNAs were measured in SRA over-expressing ECC-1 cells. Bars indicate mean ± standard deviation of three independent experiments performed in triplicate. *p < 0.05 vs. siNC. (C) Cytoplasmic and nuclear β-catenin levels in SRA knockdown ECC-1 cells were measured. (D) Cytoplasmic and nuclear β-catenin levels in SRA over-expressing ECC-1 cells were measured. (E, F) Subcellular β-catenin localization in indicated cells was assessed by immunofluorescence staining. Immunofluorescence staining of β-catenin (green) showed nuclear and cytoplasmic localization in cells transfected with siNC, siSRA, empty vector (control), or SRA over-expression. Nuclei were counterstained with DAPI (blue). Total magnification was 200×. Images were zoomed in 500%.