Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Metformin stimulated JNK/c-Jun pathway by inducing ROS generation in OS cells. (A) Intracellular ROS levels were measured by fluorescence microscope with DCFH-DA staining and (B) analyzed by flow cytometer. Scale bars = 50 μm. (C) Immunoblots of p-JNK, JNK, p-c-Jun and c-Jun protein expressions in OS cells. (D-E) Cells were incubated with metformin and were pretreated with SP600125 and NAC. Related protein expression was quantified with the help of western blot technique. *P < 0.05.
