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. 2020 Jan 1;11(1):213–228. doi: 10.7150/jca.35821

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The frequency of F4/80⁺ macrophages and CD11b⁺Ly6C⁺ cells was significantly increased in the spleens of STK24-silenced tumors. Flow cytometry was performed on the spleens of EGFP-Ctrl, sgSTK24-1.2 and sgSTK24-2.1 mice after M12 tumor cell implantation on Day 16. (A) F4/80⁺ macrophages and (B) CD11b⁺Ly6C⁺ cells were isolated from the spleens of EGFP-Ctrl and sgSTK24-2.1 mice. The numbers shown are the percentage of total cells. (C) The cell populations of CD4 T cells, CD8 T cells, F4/80⁺ macrophages, CD11b⁺ cells, and CD11b⁺Ly6C⁺ cells of splenocytes were analyzed by flow cytometry 16 days after EGFP-Ctrl and sgSTK24-1.2 tumor cell implantation. (D) The cell populations of CD4 T cells, CD8 T cells, F4/80⁺ macrophages, CD11b⁺ cells, and CD11b⁺Ly6C⁺ cells of splenocytes were analyzed by flow cytometry 16 days after EGFP-Ctrl and sgSTK24-2.1 tumor cell implantation. *P < 0.05; **P < 0.01. The results are expressed as the mean cell population ± SD, and the data are averaged from two independent experiments.