Fig 3.

Genipin enhances oxaliplatin-induced cell death via upregulating p53. (A) AGS cells were treated with genipin 100 µM for 24h. The apoptotic proteins and anti-apoptotic proteins were measured by western blotting. β-Actin was used as a loading control for each lane. (B) MKN45 cells were treated genipin for 24h. The protein expression of p53 were measured by western blotting. (C) Immunofluorescence of p53 was detected by confocal laser-scanning microscopy (original magnification: 40×). Scale bar: 5 µM (D) AGS cells were transfected with control siRNA or p53 siRNA and then the cells were treated with oxaliplatin, genipin, or combination. The activity of cleaved-PARP and cleaved-caspase 3, and cleaved-caspase 9 were measured by western blotting. (E) AGS cells were transfected with control siRNA or p53 siRNA and then the cells were treated with oxaliplatin, genipin, or combination. The cells were stained with annexin V and PI and then were measured using FACS analysis. ***P < 0.001, **P < 0.01.