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Knockdown of CDC5L inhibits cell growth of T24 and UMUC3 cells in vitro. (A) CDC5L protein expression was determined by western blot 72 h after transfection of si-CDC5L or si-NC. (B) CDC5L mRNA expression was detected by qRT-PCR 48 h after transfection of si-CDC5L or si-NC. CCK8 assay (C) and colony formation assay (D) were used to determine proliferation and colony-forming ability of si-CDC5L or si-NC-transfected T24 and UMUC3 cells. **P<0.01vs. si-NC groups (n=3).
