Figure 3.

Knockdown of CDC5L inhibits xenograft tumour growth in vivo. Tumour growth curve (A) and tumour sizes (B) after T24 cells stably expressing low levels CDC5L or empty vectors were injected into 4-week-old nude mice. (C) Fewer Ki-67+ cells and lower CDC5L expression levels were detected by IHC in sh-CDC5L treated tumours. (D) Proteins were extracted from the tumour xenografts. CDC5L, p-AKT, and AKT expression was detected by western blotting. β-actin was used as an internal control. *P<0.05, **P<0.01.