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. 2019 Nov 27;24(23):4333. doi: 10.3390/molecules24234333

Table 4.

Solvent gradient elution program used for the elution of sugars, polyols and ions. Negative values represent equilibration times.

Time [min] %A %B Flow Rate [mL min−1]
−35.000 100.00 0.000 0.300
−30.100 100.00 0.000 0.300
−30.000 100.00 0.000 0.150
−11.000 100.00 0.000 0.150
−10.000 100.00 0.000 0.300
0.000 100.00 0.000 0.300
10.000 100.00 0.000 0.300
35.000 0.000 100.00 0.300
45.000 0.000 100.00 0.300