Table 2.

Viability (%) of pistachio extracts as affected by rootstock and irrigation treatment, at 100 μg mL⁻¹ on human cancerous and non-cancerous cell lines.

Factor	HCT-116	A549	HeLa	MDA-MB-231	ARPE-19
	(% Viability)
	ANOVA 1
Rootstock	NS	***	**	NS	***
Irrigation	NS	NS	NS	NS	***
Rootstock × Irrigation	NS	***	***	NS	***
	Tukey multiple range test 2
Rootstock
P. atlantica	38.9	67.4 a	30.1 a	61.3	94.2 a
P. integerrima	39.0	68.3 a	30.1 a	61.9	87.9 b
P. terebinthus	43.4	68.7 a	32.3 a	60.3	86.6 b
CISP 3	40.2	61.1 b	28.2 b	57.2	69.2 c
Irrigation
T0	39.7	65.3	28.3	58.0	91.7 a
T1	39.8	64.4	27.0	60.8	87.7 a
T2	40.9	61.1	28.2	61.0	89.3 a
CISP 3	40.2	61.1	28.2	57.2	69.2 b
Rootstock × Irrigation
P. atlantica × T0	36.1	73.2 a	30.1 a	62.5	99.1 a
P. atlantica × T1	35.8	66.2 a	29.3 a	60.9	91.1 a
P. atlantica × T2	44.3	64.6 ab	30.4 a	59.6	90.9 a
P. integerrima × T0	36.3	74.5 a	28.4 ab	55.5	84.9 ab
P. integerrima × T1	41.0	67.2 a	31.5 a	64.6	88.3 ab
P. integerrima × T2	38.8	65.1 ab	29.6 a	64.9	90.6 a
P. terebinthus × T0	46.7	63.7 ab	34.8 a	58.9	90.1 a
P. terebinthus × T1	42.7	75.3 a	28.8 ab	59.8	83.8 ab
P. terebinthus × T2	39.9	69.1 a	33.3 a	61.3	86.2 ab
CISP 3	40.2	61.1 b	28.2 b	57.2	69.2 b
Pooled variance	4.1	2.3	1.8	4.8	4.5

^1. NS: not significant at p < 0.05; **, and ***, significant at p < 0.01, and 0.001, respectively.^2. Values (mean of 3 replications) followed by the same letter, within the same column and factor, were not significantly different (p < 0.05), Tukey’s least significant difference test. ^3. Cisplatin (CISP) concentration was 50 µg mL⁻¹ for all the cell lines studied, except for MDA-MB-231 in which case the concentration was 100 µg mL⁻¹.