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. 2019 Nov 30;24(23):4377. doi: 10.3390/molecules24234377

Figure 2.

Figure 2

Schematic of enzyme-linked aptamer sorbent assay (ELASA). In direct ELASA, the aptamer can be conjugated to an enzyme or other reporter molecule directly; in an indirect ELASA, the aptamer is first labelled with a reporter molecule, such as FITC, to which a secondary antibody-enzyme conjugate binds; in the sandwich ELASA, a biotinylated aptamer is bound to a surface to capture the target antigen, and the same aptamer-reporter molecule and secondary antibody pairing are used to provide a colourimetric change.