Figure 4.

Comparison of germinating seed proteomes 72 HAI. The principal component analysis of three replicates based on normalized profiles of over 300 proteins fulfilling stringent quantitation criteria (at two unique peptides and more than 10 matched peptide spectra) revealed a clear similarity in the eggplant seed proteome composition, indicating that the effect of temperature and/or hydrogen peroxide treatment does not drastically affect major protein components. The separation of replicate averages (± SD) highlights a distinct clustering of seeds germinated at 25 and 29 °C (separated in PC1), and a less significant separation of the hydrogen peroxide treatment in PC2. Squares and circles indicate the average of replicates and individual replicates, respectively. A two-step normalization was performed to compensate for sample loading differences and equalize the contribution of lower and high-abundant proteins.