Table 1.

Results of CPPred-RF server analysis for 70 cell-penetrating peptides (CPPs) that had high uptake efficiency with prediction confidence of above 0.9.

Peptide No.	CPPs’ Names	Amino Acid Sequence	Cell-Penetrating or Not	Prediction Confidence 1	Uptake Efficiency	Prediction Confidence 2
1	Transportan 10 (TP10)	AGYLLGKINLKALAALAKKIL	Cell-penetrating	0.98	High	1.00
2	Ala43 substitution mutant of pAntp (43–58)	AQIKIWFQNRRMKWKK	Cell-penetrating	0.95	High	0.96
3	Crot (27–39) derivative 1	CRFRFKCCKK	Cell-penetrating	0.96	High	0.98
4	Crot (27–39) derivative 2	CRFRWKCCKK	Cell-penetrating	0.96	High	0.99
5	Crot (27–39) derivative 3	CRWRFKCCKK	Cell-penetrating	0.96	High	1.00
6	CyLoP–1	CRWRWKCCKK	Cell-penetrating	0.95	High	1.00
7	Crot (27–39) derivative 4	CRWRWKCGCKK	Cell-penetrating	0.92	High	0.99
8	Crot (27–39) derivative 5	DCRWRWKCCKK	Cell-penetrating	0.82	High	0.99
9	pAntp (49–58)	FQNRRMKWKK	Cell-penetrating	0.84	High	0.91
10	Tat (48–60)	GRKKRRQRRRPPQ	Cell-penetrating	0.97	High	0.94
11	pAntp (45–58)	IKIWFQNRRMKWKK	Cell-penetrating	0.93	High	0.91
12	Bip15	IPMLK	Cell-penetrating	0.56	High	0.92
13	pAntp (47–58)	IWFQNRRMKWKK	Cell-penetrating	0.89	High	0.97
14	II	KALAALLKKLAKLLAALK	Cell-penetrating	1.00	High	0.93
15	Crot (27–39) derivative 6	KCCKWRWRCK	Cell-penetrating	0.95	High	0.94
16	Crot (27–39) derivative 7	KCGCRWRWKCGCKK	Cell-penetrating	0.95	High	0.90
17	Crot (27–39) derivative 8	KCRWRWKCCKK	Cell-penetrating	0.95	High	0.98
18	Crot (27–39) derivative 9	KDCRWRWKCCKK	Cell-penetrating	0.78	High	0.99
19	pAntp (46–58)	KIWFQNRRMKWKK	Cell-penetrating	0.93	High	0.96
20	7	KLWMRWWSPTTRRYG	Cell-penetrating	0.98	High	0.93
21	No.14–2	KLWMRWYSATTRRYG	Cell-penetrating	0.98	High	0.97
22	No.14	KLWMRWYSPTTRRYG	Cell-penetrating	0.98	High	0.96
23	No.14–7	KLWMRWYSPWTRRYG	Cell-penetrating	0.96	High	0.92
24	Crot (27–39)	KMDCRWRWKCCKK	Cell-penetrating	0.80	High	0.94
25	Crot (27–39) derivative 10	KMDCRWRWKCKK	Cell-penetrating	0.78	High	0.95
26	Crot (27–39) derivative 11	KMDCRWRWKCSKK	Cell-penetrating	0.82	High	0.95
27	Crot (27–39) derivative 12	KMDCRWRWKSCKK	Cell-penetrating	0.83	High	0.95
28	pVEC mutant 1	LLIILRARIRKQAHAHSK	Cell-penetrating	0.98	High	0.90
29	pVEC mutant 2	LLIILRRAIRKQAHAHSK	Cell-penetrating	0.98	High	0.95
30	pVEC mutant 3	LLIILRRRIRAQAHAHSK	Cell-penetrating	0.98	High	0.94
31	Crot (27–39) derivative 13	MDCRWRWKCCKK	Cell-penetrating	0.79	High	0.93
32	ARF (1-22)	MVRRFLVTLRIRRACGPPRVRV	Cell-penetrating	0.88	High	0.93
33	M918	MVTVLFRRLRIRRACGPPRVRV	Cell-penetrating	0.90	High	0.94
34	pAntp (51–58)	NRRMKWKK	Cell-penetrating	0.90	High	0.91
35	pAntp (44–58)	QIKIWFQNRRMKWKK	Cell-penetrating	0.96	High	0.92
36	pAntp (50–8)	QNRRMKWKK	Cell-penetrating	0.88	High	0.96
37	Ala44 substitution mutant of pAntp (43–58)	RAIKIWFQNRRMKWKK	Cell-penetrating	1.00	High	0.99
38	PDX -1-PTD	RHIKIWFQNRRMKWKK	Cell-penetrating	0.99	High	0.92
39	No.14–25	RLFMRFYSPTTRRYG	Cell-penetrating	0.95	High	0.93
40	No.14–17	RLWMRWASPTTRRYG	Cell-penetrating	0.99	High	0.96
41	No.14–18	RLWMRWYAPTTRRYG	Cell-penetrating	0.98	High	0.98
42	No.14–20	RLWMRWYSPATRRYG	Cell-penetrating	0.99	High	1.00
43	No.14–21	RLWMRWYSPTARRYG	Cell-penetrating	0.99	High	1.00
44	No.14–35	RLWMRWYSPTTRRYA	Cell-penetrating	0.98	High	0.98
45	No.14–1	RLWMRWYSPTTRRYG	Cell-penetrating	0.99	High	0.98
46	30	RLYMRYYSPTTRRYG	Cell-penetrating	0.97	High	0.93
47	Ala45 substitution mutant of pAntp (43–58)	RQAKIWFQNRRMKWKK	Cell-penetrating	0.98	High	0.98
48	Ala46 substitution mutant of pAntp (43–58)	RQIAIWFQNRRMKWKK	Cell-penetrating	0.98	High	0.91
49	Ala47 substitution mutant of pAntp (43–58)	RQIKAWFQNRRMKWKK	Cell-penetrating	0.99	High	0.99
50	Ala48 substitution mutant of pAntp (43–58)	RQIKIAFQNRRMKWKK	Cell-penetrating	1.00	High	0.94
51	Ala49 substitution mutant of pAntp (43–58)	RQIKIWAQNRRMKWKK	Cell-penetrating	1.00	High	0.98
52	Ala50 substitution mutant of pAntp (43–58)	RQIKIWFANRRMKWKK	Cell-penetrating	0.99	High	0.99
53	pAntpHD (Pro50)	RQIKIWFPNRRMKWKK	Cell-penetrating	0.99	High	0.96
54	Ala51 substitution mutant of pAntp (43–58)	RQIKIWFQARRMKWKK	Cell-penetrating	0.99	High	0.94
55	Ala52 substitution mutant of pAntp (43–58)	RQIKIWFQNARMKWKK	Cell-penetrating	0.95	High	0.92
56	Met-Arg	RQIKIWFQNMRRKWKK	Cell-penetrating	1.00	High	0.93
57	Ala54 substitution mutant of pAntp (43–58)	RQIKIWFQNRRAKWKK	Cell-penetrating	0.99	High	0.97
58	Penetratin	RQIKIWFQNRRMKWKK	Cell-penetrating	1.00	High	0.97
59	Retro - Tat (57–49)	RRRQRRKKR	Cell-penetrating	1.00	High	0.90
60	R6	RRRRRR	Cell-penetrating	1.00	High	0.91
61	R9	RRRRRRRRR	Cell-penetrating	1.00	High	0.91
62	Crot (27–39) derivative 14	RWRWKCCKK	Cell-penetrating	0.91	High	0.97
63	Crot (27–39) derivative 15	SRWRWKCCKK	Cell-penetrating	0.94	High	0.93
64	Rev (34–50)	TRQARRNRRRRWRERQR	Cell-penetrating	0.98	High	0.90
65	HIV-1 Rev (34–50)	TRQARRNRRRRWRERQRGC	Cell-penetrating	0.96	High	0.90
66	Bip6	VPALK	Cell-penetrating	0.74	High	0.96
67	Bip1	VPMLK	Cell-penetrating	0.57	High	0.96
68	Bip2	VPTLK	Cell-penetrating	0.67	High	0.99
69	Bip16	VPTLQ	Cell-penetrating	0.60	High	0.91
70	pAntp (48–58)	WFQNRRMKWKK	Cell-penetrating	0.84	High	0.97

¹ Prediction confidence of cell penetration. ² Prediction confidence of uptake efficiency.