Table 8.
Result of folding rate prediction using the Foldrate server and backbone flexibility analysis by the Dynamine server for CPG2 and 70 top CPPs conjugated to CPG2.
| Peptide Number | Name of the Conjugate | N-Terminal Conjugates | C-Terminal Conjugates |
|---|---|---|---|
| Predicted Half-Folding Time (sec) | Predicted Half-Folding Time (sec) | ||
| 1 | Transportan 10 (TP10)-CPG2 | 315.75 | 292.07 |
| 2 | Ala43 substitution mutant of pAntp (43-58)-CPG2 | 341.86 | 375.47 |
| 3 | Crot (27–39) derivative 1-CPG2 | 269.35 | 294.03 |
| 4 | Crot (27–39) derivative 2-CPG2 | 266.25 | 297.08 |
| 5 | Crot (27–39) derivative 3-CPG2 | 266.25 | 297.08 |
| 6 | CyLoP-1-CPG2 | 263.17 | 296.95 |
| 7 | Crot (27–39) derivative 4-CPG2 | 290.06 | 299.60 |
| 8 | Crot (27–39) derivative 5-CPG2 | 251.58 | 269.07 |
| 9 | pAntp (49–58)-CPG2 | 260.00 | 265.84 |
| 10 | Tat (48–60)-CPG2 | 250.01 | 261.29 |
| 11 | pAntp (45–58)-CPG2 | 326.14 | 336.71 |
| 12 | Bip15-CPG2 | 254.99 | 251.17 |
| 13 | pAntp (47–58)-CPG2 | 312.02 | 325.60 |
| 14 | II-CPG2 | 282.10 | 260.72 |
| 15 | Crot (27–39) derivative 6-CPG2 | 272.11 | 300.16 |
| 16 | Crot (27–39) derivative 7-CPG2 | 300.50 | 310.30 |
| 17 | Crot (27–39) derivative 8-CPG2 | 262.57 | 286.80 |
| 18 | Crot (27–39) derivative 9-CPG2 | 251.02 | 271.45 |
| 19 | pAntp (46–58)-CPG2 | 314.64 | 324.84 |
| 20 | 7-CPG2 | 304.54 | 328.42 |
| 21 | No.14–2-CPG2 | 303.00 | 306.34 |
| 22 | No.14-CPG2 | 312.62 | 340.53 |
| 23 | No.14–7-CPG2 | 288.59 | 325.49 |
| 24 | Crot (27–39)-CPG2 | 262.67 | 283.81 |
| 25 | Crot (27–39) derivative 10-CPG2 | 263.85 | 297.45 |
| 26 | Crot (27–39) derivative 11-CPG2 | 287.51 | 303.33 |
| 27 | Crot (27–39) derivative 12-CPG2 | 281.35 | 296.93 |
| 28 | pVEC mutant 1-CPG2 | 318.66 | 318.66 |
| 29 | pVEC mutant 2-CPG2 | 308.37 | 318.66 |
| 30 | pVEC mutant 3-CPG2 | 331.94 | 310.97 |
| 31 | Crot (27–39) derivative 13-CPG2 | 257.41 | 310.97 |
| 32 | ARF(1–22)-CPG2 | 375.57 | 426.04 |
| 33 | M918-CPG2 | 371.56 | 371.56 |
| 34 | pAntp (51–58)-CPG2 | 246.89 | 371.56 |
| 35 | pAntp (44–58)-CPG2 | 341.31 | 359.69 |
| 36 | pAntp (50–58)-CPG2 | 253.19 | 371.56 |
| 37 | Ala44 substitution mutant of pAntp (43–58)-CPG2 | 339.30 | 371.56 |
| 38 | PDX -1-PTD-CPG2 | 354.49 | 371.56 |
| 39 | No.14–25-CPG2 | 302.06 | 325.92 |
| 40 | No.14–17-CPG2 | 286.95 | 325.92 |
| 41 | No.14–18-CPG2 | 316.39 | 325.92 |
| 42 | No.14–20-CPG2 | 300.29 | 325.92 |
| 43 | No.14–21-CPG2 | 303.60 | 341.55 |
| 44 | No.14–35-CPG2 | 309.58 | 341.55 |
| 45 | No.14–1-CPG2 | 315.26 | 341.55 |
| 46 | 30-CPG2 | 311.23 | 328.53 |
| 47 | Ala45 substitution mutant of pAntp (43–58)-CPG2 | 321.46 | 368.26 |
| 48 | Ala46 substitution mutant of pAntp (43–58)-CPG2 | 341.11 | 368.26 |
| 49 | Ala47 substitution mutant of pAntp (43–58)-CPG2 | 301.29 | 364.51 |
| 50 | Ala48 substitution mutant of pAntp (43–58)-CPG2 | 301.06 | 364.51 |
| 51 | Ala49 substitution mutant of pAntp (43–58)-CPG2 | 328.09 | 338.69 |
| 52 | Ala50 substitution mutant of pAntp (43–58)-CPG2 | 342.91 | 357.57 |
| 53 | pAntpHD (Pro50)-CPG2 | 324.61 | 342.27 |
| 54 | Ala51 substitution mutant of pAntp (43–58)-CPG2 | 342.47 | 342.27 |
| 55 | Ala52 substitution mutant of pAntp (43–58)-CPG2 | 300.29 | 364.03 |
| 56 | Met-Arg-CPG2 | 298.31 | 315.14 |
| 57 | Ala54 substitution mutant of pAntp (43–58)-CPG2 | 295.42 | 305.35 |
| 58 | Penetratin-CPG2 | 354.39 | 400.84 |
| 59 | Retro - Tat (57–49)-CPG2 | 243.94 | 246.69 |
| 60 | R6-CPG2 | 255.43 | 249.84 |
| 61 | R9-CPG2 | 269.07 | 249.03 |
| 62 | Crot (27–39) derivative 14-CPG2 | 258.50 | 276.26 |
| 63 | Crot (27–39) derivative 15-CPG2 | 255.15 | 263.85 |
| 64 | Rev (34–50)-CPG2 | 265.86 | 251.26 |
| 65 | HIV-1 Rev (34–50)-CPG2 | 276.22 | 261.16 |
| 66 | Bip6-CPG2 | 239.77 | 256.43 |
| 67 | Bip1-CPG2 | 256.15 | 259.01 |
| 68 | Bip2-CPG2 | 240.98 | 260.66 |
| 69 | Bip16-CPG2 | 242.18 | 262.04 |
| 70 | pAntp (48–58)-CPG2 | 300.95 | 278.92 |
| CPG2 | 229.89 | 229.89 |