Figure 4.

Protection against FeNTA-induced damage in differentiated SH-SY5Y cells by thiophenols 2–6 (A) and phenols 1, 7–10 (B). After a pre-treatment with the compounds under study (10 and 50 µM) for 24 h, cells were exposed FeNTA 4 mM for additional 24 h. Cellular viability was evaluated by the MTT reduction assay. Results are expressed as the mean % of untreated controls ± SEM (n = 3). Statistical comparisons were estimated using the nonparametric method of Kruskal–Wallis (one-way ANOVA on ranks), followed by Dunn’s post hoc test (* p < 0.05 compared with cells treated only with FeNTA; ^$ p < 0.05, ^$$ p < 0.01, ^$$$ p < 0.001; ^$$$$ p < 0.0001 compared with CTRL). Positive control with caffeic acid (50 µM) was performed under the same conditions described above. The result is depicted in Supplementary Information (Figure S4B).