Table 2.

Primers used for amplification of the ToxA, ToxB and toxb genes in Ptr isolates.

Gene	Primer for singleplex PCR	Sequence	Estimated band size (bp)
ToxA	TOXA192	5′-CGT CCG GCT ACC TAG CAA TA-3′	964
	TOXA1155	5′-TTG TGC TCC TCC TTC TCG AT-3′
ToxA	ToxA1	5′-GTC ATG CGT TCT ATC CTC G-3′
	ToxA2	5′-CCT ATA GCA CCA GGT CGT CC-3′	294
ToxB	ToxB1	5′-GAC TAC CAT GCT ACT TGC TGT G-3′
	ToxB2	5′-AAC AAC GTC CTC CAC TTT GC-3′	245
toxb	90-2F1	5′-AAG TGG TCA TTG CGA CTG G-3′
	90-2R1	5′-CCT CCA CTT GCC AAA CTC TC-3′	157
Gene	Primer for multiplex PCR	Sequence	Estimated band size (bp)
CHS-1	CHS-79F	5′-TGGGGCAAGGATGCTTGGAAGAAG-3′	275
	CHS-354R	5′-TGGAAGAACCATCTGTGAGAGTTG-3′
ToxA	TA51F	5′-GCGTTCTATCCTCGTACTTC-3′	573
	TA52R	5′-GCATTCTCCAATTTTCACG-3
ToxB	TB71F	5′-GCTACTTGCTGTGGCTATC-3	232
	TB60R	5′-ACTAACAACGTCCTCCACTTTG-3′
toxb	TB71F	5′-GCTACTTGCTGTGGCTATC-3	232
	TB58R	5′-TATGAATGATTGACTGGGGTTA-3′