Figure 11.

GS-Rb1 protects H9c2 cells from apoptosis during HR. The apoptosis rate was measured by Hoechst 33342 staining. GS-Rb1 significantly reduced the number of apoptotic nuclei of H9c2 cells during HR, similar to the effect of diazoxide, compared and this effect can be eliminated by 5-HD. Cells were visualized by a fluorescent microscope (Olympus, Japan) (magnification 200x). Apoptotic nuclei display high-intensity fluorescence. HR, hypoxia-reoxygenation; GS-Rb1, ginsenoside Rb1; 5-HD, 5-hydroxydecanoate.