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. 2019 Dec 13;2019:6046405. doi: 10.1155/2019/6046405

Figure 5.

Figure 5

GS-Rb1 protects the mitochondrial membrane potential (MMP) of H9c2 cells during HR. The fluorescence intensity is negatively correlated with the MMP. (a) Control cells. (b) H9c2 cells showing high-intensity fluorescence during HR indicating reduced MMP. (c) H9c2 cells subjected to HR after pretreatment with GS-Rb1show significantly reduced fluorescence intensity compared with nontreated H9c2 cells. (d) H9c2 cells subjected to HR after simultaneous pretreatment with both GS-Rb1 and 5-HD show increased fluorescence intensity and cell rounding similar to nontreated H9c2 cells during HR. (e) H9c2 cells subjected to HR in the presence of diazoxide show reduced fluorescence similar to the protective effect seen with GS-Rb1. Cells were visualized under a fluorescent microscope (Olympus, Japan) (magnification 200×). HR, hypoxia-reoxygenation; GS-Rb1, ginsenoside Rb1; 5-HD, 5-hydroxydecanoate.