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. 2019 Dec 17;7:411. doi: 10.3389/fbioe.2019.00411

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Copyright © 2019 Lin, Li, Li, Li, Del Duke, Shen, Hao, O'Donnell, Bunnell, Goodman, Alexander, Tuan and Lin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Optimization of osteogenic medium for iMPCs seeded within mGL scaffolds. (A) Five different media are listed as Group (Gr) 1-5. The components of each medium group are listed in Table. (B) Expression of osteogenic markers in constructs treated with each medium group. All data are normalized to RPL13A. N = 4 per group; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. (C) Alizarin Red staining (top) and osteocalcin IHC (bottom) for the constructs from each group. Red (top panel) and brown (bottom panel) indicate positive staining. Bar = 200 μm.