Figure 2.

FOXD1‐AS1 promotes glioma cell proliferation and tumor growth in vitro and in vivo. A, siRNA and plasmid regulate FOXD1‐AS1 expression efficiently in human glioma cell lines. B, C, MTS and colony formation assay. FOXD1‐AS1 silencing dramatically reduced the proliferative ability of glioma cells. In contrast, FOXD1‐AS1 overexpression increased the proliferative ability of Hs683 cells. D, EdU assay. U251 and Hs683 glioma cell lines were treated with siRNA or plasmid alone, and EdU assay was performed. E, Wound healing assay. Wound healing assay indicates that FOXD1‐AS1 expression contributed to glioma cell migration. F‐H, Xenograft tumors obtained using FOXD1‐AS1 silenced cells resulted in a smaller mean volume and weight than controls. Tumors obtained using cells overexpressing FOXD1‐AS1 resulted in a larger mean volume and weight, with a more rapid development than tumors obtained from control cells. Data are presented as mean ± SEM *P < 0.05, **P < 0.01, and ***P < 0.001