Extended Data Figure 5. Alternative cleavage of RIPK1.
a, MEFs were treated with TNF (T; 10 ng/ml) combined with Smac-mimetic (S; 500 nM) for 2 hours.
b, Inducible doxycycline caspase-8-gyrase41, mouse RIPK1 wild type and mutants constructs or GFP were co-expressed in 293T. Cells were treated for 2 hours with 1 μg/ml doxycycline to induce caspase-8-gyrase expression and then for 2 hours with 700 nM coumermycin to dimerize caspase-8-gyrase. Antibody recognising the N-terminal end of RIPK1 was used. Results are representative of 2 a, b, Results are representative of 4 (a) and 2 (b) independent experiments. For gel source data, see Supplementary Figure 2.