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. 2019 Dec 19;10:1427. doi: 10.3389/fphar.2019.01427

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Copyright © 2019 He, Law, Zhang, Qiu, Qu, Wu, Han, Song, Zheng, Liu, He and Wong

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Bavachin activates autophagy via mTOR signaling in HASMCs. (A) Western blot analysis of mTOR and autophagy-related proteins (p62, beclin1, and LC3) in bavachin and β-GP-treated HASMCs. The full-length images of Western blot are shown in Figure S5A . (B) Immunofluorescence analysis of LC3-II puncta formation (red color represents LC3-II, blue represents DAPI; scale bar = 25 μm). Statistical significance was analyzed by the one-way ANOVA (*P < 0.05, **P < 0.01, ***P < 0.001). The data is represented as mean ± S.D. (n = 3).