FIGURE 3.
Developmental and tissue-specific expression patterns of SfVg and SfVgR. Relative expression levels of SfVg (A) and SfVgR (D) in the first to fifth nymphal instars (N1, N2, N3, N4, N5), and adults, females and males; Relative expression levels of SfVg (B) and SfVgR (E) in the females at different time after emergence; The transcript levels of SfVg (C) and SfVgR (F) in various tissues of females. HAE, hours after emergence; Mg, midgut; Ov, ovary; Hd, head; Th, thorax; Fb, fat body. The bar means the average (±SE) of three biological repetitions. S. furcifera α-1 tubulin (SfTub) and elongation factor 1α (SfEF1α) were used as internal control. Different letters above the bars represent significant differences (ANOVA followed by Tukey’s test, P < 0.05).