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. 2019 Dec 19;10:1532. doi: 10.3389/fphys.2019.01532

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Copyright © 2019 Hu, Tian, Tang, Yang, Qiu, He, Ding, Li and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Functional analysis of SfVg and SfVgR in the ovarian development of S. furcifera females. Expression levels of SfVg (A) and SfVgR (B) in females after injected with dsVg, dsVgR and dsEGFP for 48, 72, and 96 h. Asterisk indicate statistically significant difference (t-test: ^∗P < 0.05, ^∗∗P < 0.01, ^∗∗∗P < 0.001). “ns” indicates no significant difference. (C) Effect of SfVg (dsVg) and SfVgR (dsVgR) RNAi on ovarian development with dsEGFP as a control. Ovaries were dissected at 132 h post-injection. SfEF1a and SfTub were used as internal control. The red, white, blue and yellow arrowheads marked the complete oocytes, incomplete oocytes, ovarioles and oviducts, respectively. Scale bar, 0.5 mm.