. 2019 Dec 19;10:1532. doi: 10.3389/fphys.2019.01532

TABLE 1.

Primers used in this study.

Purpose	Primer name	Primer sequence (5′→3′)	E (%)^a	R²
qPCR	qVg-F	CACAAGGTTGCTTCTGGCATC	93.8	0.999
	qVg-R	TTGGCCAAAGCTAGAGTAGCC
	qVgR-F	ACAAGAGCGATCCTGCCAAA	96.3	0.993
	qVgR-R	ATTCGATCCACTCGTGTCCG
	qTub-F	GAGGACACTACACCATCGGC	93.6	0.995
	qTub-R	TCAACAGCGAGGTGAATCCG
	qEF1α-F	AAGATCGGTTACAACCCGGC	103.8	0.989
	qEF1α-R	TCCTTGCGCTCAATGTTCCA
RNAi	Vg-F	GGATCCTAATACGACTCACTATAGGAGGGCTTTGGAGATCTTGCC^b	n.a.	n.a.
	Vg-R	GGATCCTAATACGACTCACTATAGGAGAGTTGGCTGGGTCCATTG
	VgR-F	GGATCCTAATACGACTCACTATAGGACCACAGCCACCAACGATAG	n.a.	n.a.
	VgR-R	GGATCCTAATACGACTCACTATAGGACGTCAGGGGACGTAAACAC
	EGFP-F	GGATCCTAATACGACTCACTATAGGGAGGACGACGGCAACTACAAG	n.a.	n.a.
	EGFP-R	GGATCCTAATACGACTCACTATAGGGGTCCATGCCGAGAGTGATCC

^aPCR efficiency; ^bT7 RNA polymerase promoter is underlined; n.a. = not applicable.