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. 2019 Dec 19;10:2979. doi: 10.3389/fimmu.2019.02979

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Copyright © 2019 Ishisono, Mano, Yabe and Kitaguchi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of pectin pre-treatment on interleukin (IL)-6 production in RAW264.7 macrophages. Cells were pre-treated with 250 μg/mL citrus or orange pectin for 24 h and stimulated with 1 μg/mL (A) Pam3CSK4 or (B) LPS. (C) Cells were pre-treated with 50 μg/mL polysaccharides derived from side chain of citrus or orange pectin for 24 hours and stimulated with 1 μg/mL LPS. The IL-6 concentration in the supernatant was determined 24 h after toll-like receptor stimulation. Values are presented as means ± standard error of the mean of three independent experiments. Values not sharing a common letter (a or b) were significantly different (P < 0.05).

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