Figure 3.

Increased glomerular volume and glomerulosclerosis in UNx db/db mice. (a) Representative images of PAS‐stained kidney sections from female Sham db/+ and UNx db/db mice. The arrows represent dilated tubules. Scale bar 100 µm. (b) Total glomerular volume quantified by stereology on PAS‐stained kidney sections at week 16. (c) A double immunohistochemical stain for podocin (green) and collagen IV (yellow) was performed to quantify glomerulosclerosis (white overlay). (d) Total glomerular collagen IV was quantified using stereology on podocin/collagen IV double stained kidney sections at week 16. Values represent mean + SEM in Sham db/+, male (n = 5), UNx db/db, male (n = 12), Sham db/+, female (n = 5–8) and UNx db/db female (n = 10); *p < .05, **p < .01, ***p < .001 UNx db/db versus Sham db/+