Skip to main content
. 2019 Dec 19;10:2847. doi: 10.3389/fmicb.2019.02847

FIGURE 4.

FIGURE 4

FHbp with SP SNPs shows reduced binding to SecA. Bacterial two hybrid experiments to compare FHbp binding to SecA in MC58 and L91543. The fHbp gene of strains MC58 and L91543 and secA gene of MC58 were cloned into the two-hybrid pUT18 (T18-prey) and pKT25 (T25-bait) vectors respectively and the appropriate prey and bait pair of vectors co-transformed in BTH101. In parallel, zip prey and zip bait vectors were co-transformed in BTH101 as a positive control for protein–protein interaction. Likewise, different prey-bait combinations but with one vector or both vectors empty were used as negative controls. The interactions were quantified by measuring the β-galactosidase level expressed in Miller units (values taken from four independent clones). Unpaired t-tests with Welsch’s correction were conducted to compare the interactions. All columns represent mean ± SEM, ∗p < 0.05 vs. MC58 FHbp.