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. 2019 Dec 19;10:2847. doi: 10.3389/fmicb.2019.02847

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Copyright © 2019 da Silva, Karlyshev, Oldfield, Wooldridge, Bayliss, Ryan and Griffin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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FHbp with SP SNPs localize to the surface via Lnt and Slam with escape from processing by Lgt and LspA. (A) Western immunoblot after growing strains in palmitic acid alkyne and clicking immuno-precipitated FHbp with biotin-azide; Streptavidin-HRP (upper panel) and JAR4 (lower panel). (B,C) Immuno-dot blot of broth cultures (A₆₀₀ 0.5) and Western immunoblot of WCL with JAR4. For immuno-dot blot densitometer values, unpaired t-tests with Welsch’s correction were conducted to compare the isogenic strains. All columns represent mean ± SEM, ^∗p < 0.05 vs. wild type isogenic strain. (D) Western immunoblot of different cellular fractions with JAR4. (Lane 1, MC58; 2, L91543; 3, isolate 6; 4, isolate 18.) (B–D) Equal protein loading was confirmed by the determination of RecA protein in each sample.