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. 2019 Dec 19;10:2847. doi: 10.3389/fmicb.2019.02847

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Copyright © 2019 da Silva, Karlyshev, Oldfield, Wooldridge, Bayliss, Ryan and Griffin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The affect of increasing transcription of fHbp on FHbp subcellular localization and surface localization in L91543. (A) Immuno-dot blot of whole cells with JAR4. Lanes; 1, MC58, 2, L91543, 3, L91543ΔfHbp + LfHbp induced with IPTG, 4, L91543ΔfHbp + LfHbp non-induced. One representative experiment of 5 is shown. The optical density was measured by ImageJ. The ANOVA followed by Dunnett’s test was employed for statistical analysis in GraphPad (V. 6). All columns represent mean ± SEM, ^∗p < 0.05 vs. strain L91543. (B) Western immunoblot with JAR4 of different subcellular compartments of strain L91543ΔfHbp + LfHbp non-induced versus induced. Equal protein loading was confirmed by the determination of RecA protein.