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. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: Methods Enzymol. 2018 Dec 1;616:337–363. doi: 10.1016/bs.mie.2018.10.026

Fig. 6.

Fig. 6

Effect of nonspecific RNA competitors on formation of Cas9/sgRNA complexes. (A and B) Time dependencies of the increase in fluorescence upon the addition of 5nM(A) or 50nM (B) sgRNAs to samples containing Cas9, beacon, and 0.05mg/mL yeast tRNA; (C) Time dependence of the increase in fluorescence upon the addition of 5nM sgRNAs to samples containing Cas9, beacon, and 0.01mg/mL total RNA from human lung.